Oligo synthesis company, oligo synthesis services
As with any other organic compound, it is prudent to characterize synthetic oligonucleotides upon their preparation. Non-nucleoside phosphoramidites are the phosphoramidite reagents designed to introduce various functionalities at the termini of synthetic oligonucleotides or between nucleotide residues in the middle of the sequence. Oxidation[ edit ] The newly formed tricoordinated phosphite triester linkage is not natural and is of limited stability under the conditions of oligonucleotide synthesis.
The replacement of the non-bridging oxygen with sulfur creates a new center of chirality at phosphorus. This property determines the use essay evaluating student OPS as compare and contrast essay prompts for high school oligonucleotides in in vitro and in vivo applications where the extensive exposure to nucleases is inevitable. Similarly to non-nucleosidic phosphoramidites, many other synthesis solid supports designed for the attachment of reactive functional groups, non-radioactive reporter groups, and terminal modifiers e.
The initial platform called the VLSS for very large scale synthesizer utilized large Pharmacia liquid chromatograph columns as reactors and could synthesize up to 75 millimoles of material.
In most cases all that was required was to augment the number of fluids that could be delivered to the column. In more complex cases research and large company syntheses oligonucleotides are characterized after their deprotection and after purification.
Synthesis Strategy The most frequently used oligonucleotide synthesis strategy is based upon beta-cyanoethyl phosphoramidite chemistry.
However, the applicability of this method is limited by the fact that the cleavage of 2-cyanoethyl phosphate protection gives rise to acrylonitrile as a side product. Oligonucleotide synthesis involved the use of CPG controlled pore glass which is a rigid support and is more suited for column reactors as described above.
PerSeptive Biosystems as well as Pharmacia GE were two of several companies that developed synthesizers out of liquid chromatographs.